Amoroso, G and Ventura, T and Adams, M and Carter, CG and Battaglene, SC and Elizur, A and Cobcroft, JM, Lower jaw deformity in triploid Atlantic salmon: an integrated morphological and transcriptomic investigation, Interdisciplinary Approaches in Fish Skeletal Biology Abstracts, 27-30 April, Tavira, Algarve, Portugal (2015) [Conference Extract]
Our study assessed the effect of ploidy (diploid and triploid) and temperature (14 and 18°C) on the prevalence of several skeletal deformities in all-female Atlantic salmon from 3 to 60 g. The prevalence of LJD was confirmed to be higher in triploids and no temperature effect was observed. Samples of LJD-affected and morphologically normal triploid fish were collected for molecular analysis at the end of the developmental assessment (57.2 ± 1.4 g) when the prevalence of LJD was 10.9 ± 3.5%.
Recently, LJD occurrence has been linked to phosphorus deficiency in the diet of triploid salmon (Baeverfjord et al., 2009; Fjelldal et al., 2015). Little is known about the mechanisms underlying LJD in triploids, so we took a transcriptome-wide descriptive approach to examine changes in gene expression in the jaw of normal and LJD-affected triploid fish. In addition, we performed RT-qPCR to test the effect of temperature (standard and high) in normal and LJD-affected triploid fish on the expression of transcripts known to be related to bone and cartilage (i.e. alp, col1a1, mmp13, osteocalcin, trap and col2a1).
Analysis of our transcriptomic database implicated numerous genes encoding fundamental structural proteins in LJD, suggesting it is a multigenic-regulated trait. Characterisation and analysis of known roles of the differentially expressed genes suggests that an impairment in cartilage formation might underlie the predisposition to develop LJD, which could be exacerbated by dietary phosphorus deficiency.
|Item Type:||Conference Extract|
|Keywords:||Atlantic salmon, jaw deformity|
|Research Division:||Agricultural and Veterinary Sciences|
|Research Group:||Fisheries Sciences|
|Objective Division:||Animal Production and Animal Primary Products|
|Objective Group:||Fisheries - Aquaculture|
|Objective Field:||Aquaculture Fin Fish (excl. Tuna)|
|Author:||Amoroso, G (Mr Gianluca Amoroso)|
|Author:||Adams, M (Dr Mark Adams)|
|Author:||Carter, CG (Professor Chris Carter)|
|Author:||Battaglene, SC (Associate Professor Stephen Battaglene)|
|Author:||Cobcroft, JM (Dr Jennifer Cobcroft)|
|Deposited By:||Sustainable Marine Research Collaboration|
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