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Knockout of the two evolutionarily conserved peroxisomal 3-ketoacyl-CoA thiolases in Arabidopsis recapitulates the abnormal inflorescence meristem 1 phenotype
Citation
Wiszniewski, AAG and Bussell, JD and Long, RL and Smith, SM, Knockout of the two evolutionarily conserved peroxisomal 3-ketoacyl-CoA thiolases in Arabidopsis recapitulates the abnormal inflorescence meristem 1 phenotype, Journal of Experimental Botany, 65, (22) pp. 6723-6733. ISSN 0022-0957 (2014) [Refereed Article]
 | PDF (Knockout of the two evolutionarily conserved peroxisomal 3-ketoacyl-CoA thiolases in Arabidopsis recapitulates the abnormal inflorescence meristem 1 phenotype) 2Mb |
Copyright Statement
Copyright 2014 The Author Licenced under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/
Abstract
A specific function for peroxisomal β-oxidation in inflorescence development in Arabidopsis thaliana is suggested by
the mutation of the ABNORMAL INFLORESCENCE MERISTEM 1 gene, which encodes one of two peroxisomal multifunctional
proteins. Therefore, it should be possible to identify other β-oxidation mutants that recapitulate the aim1
phenotype. Three genes encode peroxisomal 3-ketoacyl-CoA thiolase (KAT) in Arabidopsis. KAT2 and KAT5 are present
throughout angiosperms whereas KAT1 is a Brassicaceae-specific duplication of KAT2 expressed at low levels in
Arabidopsis. KAT2 plays a dominant role in all known aspects of peroxisomal β-oxidation, including that of fatty acids,
pro-auxins, jasmonate precursor oxophytodienoic acid, and trans-cinnamic acid. The functions of KAT1 and KAT5 are
unknown. Since KAT5 is conserved throughout vascular plants and expressed strongly in flowers, kat2 kat5 double
mutants were generated. These were slow growing, had abnormally branched inflorescences, and ectopic organ
growth. They made viable pollen, but produced no seed indicating that infertility was due to defective gynaecium
function. These phenotypes are strikingly similar to those of aim1. KAT5 in the Brassicaceae encodes both cytosolic
and peroxisomal proteins and kat2 kat5 defects could be complemented by the re-introduction of peroxisomal (but
not cytosolic) KAT5. It is concluded that peroxisomal KAT2 and KAT5 have partially redundant functions and operate
downstream of AIM1 to provide β-oxidation functions essential for inflorescence development and fertility.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | 3-Ketoacyl-CoA thiolase, Arabidopsis thaliana, β-oxidation, flowering, germination, peroxisome, seed development |
| Research Division: | Biological Sciences |
| Research Group: | Plant Biology |
| Research Field: | Plant Physiology |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Biological Sciences |
| UTAS Author: | Smith, SM (Professor Steven Smith) |
| ID Code: | 101315 |
| Year Published: | 2014 |
| Web of Science® Times Cited: | 11 |
| Deposited By: | Plant Science |
| Deposited On: | 2015-06-17 |
| Last Modified: | 2017-11-02 |
| Downloads: | 393 View Download Statistics |
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