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Neoparamoeba perurans loses virulence during clonal culture

Citation

Bridle, AR and Davenport, DL and Crosbie, PBB and Polinski, M and Nowak, BF, Neoparamoeba perurans loses virulence during clonal culture, International Journal for Parasitology, 45, (9-10) pp. 575-578. ISSN 0020-7519 (2015) [Refereed Article]


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Copyright Statement

Copyright 2015 Australian Society for Parasitology Inc.

DOI: doi:10.1016/j.ijpara.2015.04.005

Abstract

Amoebic Gill Disease affects farmed salmonids and is caused by Neoparamoeba perurans. Clonal cultures of this amoeba have been used for challenge experiments, however the effect of long-term culture on virulence has not been investigated. Here we show, using in vitro and in vivo methods, that a clone of N. perurans which was virulent 70 days after clonal culture lost virulence after 3 years in clonal culture. We propose that this is related either to the lack of attachment to the gills or the absence of an extracellular product, as shown by the lack of cytopathic effect on Chinook salmon embryo cells. The avirulent clonal culture of N. perurans allowed us to propose two potential virulence mechanisms/factors involved in Amoebic Gill Disease and is an invaluable tool for host-pathogen studies of Amoebic Gill Disease.

Item Details

Item Type:Refereed Article
Keywords:amoeba, salmon, gill, aquaculture, clone, virulence, Amoebic Gill Disease, AGD, Salmo salar
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Bridle, AR (Dr Andrew Bridle)
Author:Davenport, DL (Ms Danielle Davenport)
Author:Crosbie, PBB (Dr Philip Crosbie)
Author:Polinski, M (Mr Mark Polinski)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:101272
Year Published:2015
Web of Science® Times Cited:7
Deposited By:IMAS Research and Education Centre
Deposited On:2015-06-16
Last Modified:2017-11-07
Downloads:222 View Download Statistics

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