University of Tasmania
Browse
575 Bridle.pdf (560.66 kB)

Neoparamoeba perurans loses virulence during clonal culture

Download (560.66 kB)
journal contribution
posted on 2023-05-18, 11:02 authored by Andrew BridleAndrew Bridle, Davenport, D, Crosbie, PBB, Polinski, M, Barbara NowakBarbara Nowak
Amoebic Gill Disease affects farmed salmonids and is caused by Neoparamoeba perurans. Clonal cultures of this amoeba have been used for challenge experiments, however the effect of long-term culture on virulence has not been investigated. Here we show, using in vitro and in vivo methods, that a clone of N. perurans which was virulent 70 days after clonal culture lost virulence after 3 years in clonal culture. We propose that this is related either to the lack of attachment to the gills or the absence of an extracellular product, as shown by the lack of cytopathic effect on Chinook salmon embryo cells. The avirulent clonal culture of N. perurans allowed us to propose two potential virulence mechanisms/factors involved in Amoebic Gill Disease and is an invaluable tool for host-pathogen studies of Amoebic Gill Disease.

History

Publication title

International Journal for Parasitology

Volume

45

Issue

9-10

Pagination

575-578

ISSN

0020-7519

Department/School

Institute for Marine and Antarctic Studies

Publisher

Pergamon-Elsevier Science Ltd

Place of publication

The Boulevard, Langford Lane, Kidlington, Oxford, England, Ox5 1Gb

Rights statement

Copyright 2015 Australian Society for Parasitology Inc.

Repository Status

  • Open

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

Usage metrics

    University Of Tasmania

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC