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Amoebic Gill Disease affects farmed salmonids and is caused by Neoparamoeba perurans. Clonal cultures of this amoeba have been used for challenge experiments, however the effect of long-term culture on virulence has not been investigated. Here we show, using in vitro and in vivo methods, that a clone of N. perurans which was virulent 70 days after clonal culture lost virulence after 3 years in clonal culture. We propose that this is related either to the lack of attachment to the gills or the absence of an extracellular product, as shown by the lack of cytopathic effect on Chinook salmon embryo cells. The avirulent clonal culture of N. perurans allowed us to propose two potential virulence mechanisms/factors involved in Amoebic Gill Disease and is an invaluable tool for host-pathogen studies of Amoebic Gill Disease.

Item Type:	Refereed Article
Keywords:	amoeba, salmon, gill, aquaculture, clone, virulence, Amoebic Gill Disease, AGD, Salmo salar
Research Division:	Agricultural and Veterinary Sciences
Research Group:	Fisheries Sciences
Research Field:	Aquaculture
Objective Division:	Animal Production and Animal Primary Products
Objective Group:	Fisheries - Aquaculture
Objective Field:	Fisheries - Aquaculture not elsewhere classified
UTAS Author:	Bridle, AR (Dr Andrew Bridle)
UTAS Author:	Davenport, DL (Ms Danielle Davenport)
UTAS Author:	Crosbie, PBB (Dr Philip Crosbie)
UTAS Author:	Polinski, M (Mr Mark Polinski)
UTAS Author:	Nowak, BF (Professor Barbara Nowak)
ID Code:	101272
Year Published:	2015
Web of Science® Times Cited:	11
Deposited By:	IMAS Research and Education Centre
Deposited On:	2015-06-16
Last Modified:	2017-11-07
Downloads:	241 View Download Statistics