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Neoparamoeba perurans loses virulence during clonal culture
Citation
Bridle, AR and Davenport, D and Crosbie, PBB and Polinski, M and Nowak, BF, Neoparamoeba perurans loses virulence during clonal culture, International Journal for Parasitology, 45, (9-10) pp. 575-578. ISSN 0020-7519 (2015) [Refereed Article]
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Copyright Statement
Copyright 2015 Australian Society for Parasitology Inc.
DOI: doi:10.1016/j.ijpara.2015.04.005
Abstract
Amoebic Gill Disease affects farmed salmonids and is caused by Neoparamoeba perurans. Clonal cultures
of this amoeba have been used for challenge experiments, however the effect of long-term culture on
virulence has not been investigated. Here we show, using in vitro and in vivo methods, that a clone of
N. perurans which was virulent 70 days after clonal culture lost virulence after 3 years in clonal culture.
We propose that this is related either to the lack of attachment to the gills or the absence of an extracellular
product, as shown by the lack of cytopathic effect on Chinook salmon embryo cells. The avirulent
clonal culture of N. perurans allowed us to propose two potential virulence mechanisms/factors involved
in Amoebic Gill Disease and is an invaluable tool for host-pathogen studies of Amoebic Gill Disease.
Item Details
Item Type: | Refereed Article |
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Keywords: | amoeba, salmon, gill, aquaculture, clone, virulence, Amoebic Gill Disease, AGD, Salmo salar |
Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Aquaculture |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - aquaculture |
Objective Field: | Fisheries - aquaculture not elsewhere classified |
UTAS Author: | Bridle, AR (Associate Professor Andrew Bridle) |
UTAS Author: | Davenport, D (Miss Danielle Davenport) |
UTAS Author: | Crosbie, PBB (Dr Philip Crosbie) |
UTAS Author: | Polinski, M (Mr Mark Polinski) |
UTAS Author: | Nowak, BF (Professor Barbara Nowak) |
ID Code: | 101272 |
Year Published: | 2015 |
Web of Science® Times Cited: | 30 |
Deposited By: | IMAS Research and Education Centre |
Deposited On: | 2015-06-16 |
Last Modified: | 2017-11-07 |
Downloads: | 452 View Download Statistics |
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