Ye, L and Huang, Y and Dai, F and Ning, H and Li, C and Zhou, M and Zhang, G, Identification of two key genes controlling chill haze stability of beer in barley (Hordeum vulgare L), BMC Genomics, 16 Article 449. ISSN 1471-2164 (2015) [Refereed Article]
Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/
Background: In bright beer, haze formation is a serious quality problem, degrading beer quality and reducing its shelf life. The quality of barley (Hordeum vulgare L) malt, as the main raw material for beer brewing, largely affects the colloidal stability of beer.
Results: In this study, the genetic mechanism of the factors affecting beer haze stability in barley was studied. Quantitative trait loci (QTL) analysis of alcohol chill haze (ACH) in beer was carried out using a Franklin/Yerong double haploid (DH) population. One QTL, named as qACH, was detected for ACH, and it was located on the position of about 108 cM in chromosome 4H and can explain about 20 % of the phenotypic variation. Two key haze active proteins, BATI-CMb and BATI-CMd were identified by proteomics analysis. Bioinformatics analysis showed that BATI-CMb and BATI-CMd had the same position as qACH in the chromosome. It may be deduced that BATI-CMb and BATI-CMd are candidate genes for qACH, controlling colloidal stability of beer. Polymorphism comparison between Yerong and Franklin in the nucleotide and amino acid sequence of BATI-CMb and BATI-CMd detected the corresponding gene specific markers, which could be used in marker-assisted selection for malt barley breeding.
Conclusions: We identified a novel QTL, qACH controlling chill haze of beer, and two key haze active proteins, BATI-CMb and BATI-CMd. And further analysis showed that BATI-CMb and BATI-CMd might be the candidate genes associated with beer chill haze.
|Item Type:||Refereed Article|
|Keywords:||barley, malting quality, QTL, beer haze, malt, quantitative trait loci (QTL)|
|Research Division:||Agricultural and Veterinary Sciences|
|Research Group:||Crop and Pasture Production|
|Research Field:||Crop and Pasture Improvement (Selection and Breeding)|
|Objective Division:||Plant Production and Plant Primary Products|
|Objective Group:||Winter Grains and Oilseeds|
|UTAS Author:||Zhou, M (Professor Meixue Zhou)|
|Web of Science® Times Cited:||5|
|Deposited By:||Tasmanian Institute of Agriculture|
|Downloads:||166 View Download Statistics|
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