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Waterlogging tolerance in barley is associated with faster aerenchyma formation in adventitious roots


Zhang, X and Shabala, S and Koutoulis, A and Shabala, L and Johnson, P and Hayes, D and Nichols, DS and Zhou, M, Waterlogging tolerance in barley is associated with faster aerenchyma formation in adventitious roots, Plant and Soil, 394, (1) pp. 355-372. ISSN 0032-079X (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Springer International

DOI: doi:10.1007/s11104-015-2536-z


Background and aims Plant adaptation to waterlogged conditions requires a set of morphological and physiological/biochemical changes. The formation of aerenchyma is one of the most crucial adaptive traits for waterlogging tolerance. Enzymatic scavenging may also potentially contribute to waterlogging tolerance by providing detoxification of reactive oxygen species (ROS).

Methods Changes of root porosity (as an indicator of aerenchyma formation) and activities in leaves of four major antioxidant enzymes, γ-amino butyric acid (GABA) and lactic acid contents in roots were evaluated in six barley genotypes contrasting in waterlogging tolerance.

Results Soil waterlogging caused significant increases in adventitious root porosity in all genotypes. Waterlogging-tolerant genotypes showed not only significantly higher adventitious root porosity than sensitive genotypes but also much faster development of aerenchyma. The greatest difference in adventitious root porosity among genotypes was observed after 7 days of waterlogging treatment. At the same time, antioxidant enzyme activities in leaves, GABA and lactic acid contents in roots did not correlate with waterlogging tolerance.

Conclusions A faster formation of aerenchyma in adventitious roots is one of the key factors for waterlogging tolerance in barley. This protocol is recommended to be applied in future studies to identify molecular markers linked to this trait using appropriate mapping populations.

Item Details

Item Type:Refereed Article
Keywords:barley, waterlogging, aerenchyma
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Barley
UTAS Author:Zhang, X (Mr Xuechen Zhang)
UTAS Author:Shabala, S (Professor Sergey Shabala)
UTAS Author:Koutoulis, A (Professor Anthony Koutoulis)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Johnson, P (Dr Peter Johnson)
UTAS Author:Nichols, DS (Dr David Nichols)
UTAS Author:Zhou, M (Professor Meixue Zhou)
ID Code:101029
Year Published:2015
Funding Support:Australian Research Council (LP120200516)
Web of Science® Times Cited:5
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-06-08
Last Modified:2017-11-06

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