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Genetic diversity and QTL mapping of thermostability of limit dextrinase in barley
journal contribution
posted on 2023-05-18, 10:42 authored by Wang, X, Zhang, X, Cai, S, Ye, L, Meixue ZhouMeixue Zhou, Chen, Z, Zhang, G, Dai, FLimit dextrinase (LD) is an essential amylolytic enzyme for the complete degradation of starch, and it is closely associated with malt quality. A survey of 51 cultivated barley and 40 Tibetan wild barley genotypes showed a wide genetic diversity of LD activity and LD thermostability. Compared with cultivated barley, Tibetan wild barley showed lower LD activity and higher LD thermostability. A doubled haploid population composed of 496 DArT and 28 microsatellite markers was used for mapping Quantitative Trait Loci (QTLs). Parental line Yerong showed low LD activity and high LD thermostability, but Franklin exhibited high LD activity and low LD thermostability. Three QTLs associated with thermostable LD were identified. The major QTL is close to the LD gene on chromosome 7H. The two minor QTLs colocalized with previously reported QTLs determining malt-extract and diastatic power on chromosomes 1H and 2H, respectively. These QTLs may be useful for a better understanding of the genetic control of LD activity and LD thermostability in barley.
Funding
Grains Research & Development Corporation
History
Publication title
Journal of Agricultural and Food ChemistryVolume
63Issue
14Pagination
3778-3783ISSN
0021-8561Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Amer Chemical SocPlace of publication
1155 16Th St, Nw, Washington, USA, Dc, 20036Rights statement
© 2015 American Chemical SocietyRepository Status
- Restricted