Limit dextrinase (LD) is an essential amylolytic enzyme for the complete degradation of starch, and it is closely associated with malt quality. A survey of 51 cultivated barley and 40 Tibetan wild barley genotypes showed a wide genetic diversity of LD activity and LD thermostability. Compared with cultivated barley, Tibetan wild barley showed lower LD activity and higher LD thermostability. A doubled haploid population composed of 496 DArT and 28 microsatellite markers was used for mapping Quantitative Trait Loci (QTLs). Parental line Yerong showed low LD activity and high LD thermostability, but Franklin exhibited high LD activity and low LD thermostability. Three QTLs associated with thermostable LD were identified. The major QTL is close to the LD gene on chromosome 7H. The two minor QTLs colocalized with previously reported QTLs determining malt-extract and diastatic power on chromosomes 1H and 2H, respectively. These QTLs may be useful for a better understanding of the genetic control of LD activity and LD thermostability in barley.

Item Type:	Refereed Article
Keywords:	barley, Hordeum vulgare L., malting quality, QTL, Tibetan wild barley, limit dextrinase, thermostability, quantitative trait locus
Research Division:	Agricultural and Veterinary Sciences
Research Group:	Crop and Pasture Production
Research Field:	Crop and Pasture Improvement (Selection and Breeding)
Objective Division:	Plant Production and Plant Primary Products
Objective Group:	Winter Grains and Oilseeds
Objective Field:	Barley
UTAS Author:	Zhou, M (Professor Meixue Zhou)
ID Code:	101028
Year Published:	2015
Web of Science® Times Cited:	5
Deposited By:	Tasmanian Institute of Agriculture
Deposited On:	2015-06-08
Last Modified:	2017-11-06
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