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Kallikrein 4 (KLK4), A New Member of the Human Kallikrein Gene Family Is Upregulated By Estrogen and Progesterone in the Human Endometrial Cancer Cell Line, KLE

Citation

Myers, SA and Clements, JA, Kallikrein 4 (KLK4), A New Member of the Human Kallikrein Gene Family Is Upregulated By Estrogen and Progesterone in the Human Endometrial Cancer Cell Line, KLE, The Journal of Clinical Endocrinology & Metabolism, 86, (5) pp. 2323-2326. ISSN 0021-972X (2001) [Refereed Article]


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Copyright Statement

Copyright 2001 The Endocrine Society

DOI: doi:10.1210/jc.86.5.2323

Abstract

Endometrial cancer is the fourth most common female malignancy in women in developed countries. Estrogen, and to a lesser degree, progesterone, regulate specific target genes that are involved in endometrial tumorigenesis. A family of proteases involved in cellular proliferation, extracellular matrix degradation and thus, implicated in tumorigenesis, and regulated by estrogen and progesterone in a number of systems, are the tissue kallikreins (KLKs). KLK4, a new member of the KLK gene family, was found to be expressed to varying levels in a number of endometrial cancer cell lines- HEC1A, HEC1B, Ishikawa, RL95-2 and KLE- at both the mRNA and protein level. On the addition of 10 nmol/L estradiol, progesterone, or a combination of both over a 48 h period, an increase in the intracellular protein levels of K4 were observed when compared to the control (untreated) cells. We have also identified a novel KLK4 transcript with a complete exon 4 deletion. The significance of this alternative transcript, which would give rise to a truncated protein without a serine residue (which is essential for catalytic activity), is yet to be established. These cell lines now provide a model system to study the role of KLK4 and the molecular mechanisms of KLK4 regulation by estrogen and progesterone, in endometrial tumorigenesis.

Item Details

Item Type:Refereed Article
Keywords:Kallikrein, Breast and Prostate cancer
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Genomics
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Myers, SA (Dr Stephen Myers)
ID Code:100908
Year Published:2001
Web of Science® Times Cited:42
Deposited By:Health Sciences B
Deposited On:2015-06-03
Last Modified:2015-06-15
Downloads:130 View Download Statistics

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