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Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach


Zivanovic, BD and Shabala, L and Elzenga, TJM and Shabala, SN, Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach, Planta, 242, (4) pp. 813-827. ISSN 0032-0935 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Springer-Verlag Berlin Heidelberg

DOI: doi:10.1007/s00425-015-2316-2


Blue light signalling pathway in epidermal tissue isolated from the abaxial side of fully developed Vicia faba leaves was dissected by measuring the effect of inhibitors of second messengers on net K+, Ca2+ and H+ fluxes using non-invasive ion-selective microelectrodes (the MIFE system). Switching the blue light on-off caused transient changes of the ion fluxes. The effects of seven groups of inhibitors were tested in this study: CaM antagonists, ATPase inhibitors, Ca2+ anatagonists or chelators, agents affecting IP3 formation, redox system inhibitors, inhibitors of endomembrane Ca2+ transport systems and an inhibitor of plasma membrane Ca2+-permeable channels. Most of the inhibitors had a significant effect on steady-state (basal) net fluxes, as well as on the magnitude of the transient ion flux responses to blue light fluctuations. The data presented in this study suggest that redox signalling and, specifically, plasma membrane NADPH oxidase and coupled Ca2+ and K+ fluxes play an essential role in blue light signal transduction.

Item Details

Item Type:Refereed Article
Keywords:broad bean;inhibitors;ion fluxes;microelectrodes
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Grain legumes
UTAS Author:Zivanovic, BD (Dr Branka Zivanovic)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Shabala, SN (Professor Sergey Shabala)
ID Code:100733
Year Published:2015
Web of Science® Times Cited:3
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-05-27
Last Modified:2022-08-18

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