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Developing and validating a high-throughput assay for salinity tissue tolerance in wheat and barley

Citation

Wu, H and Shabala, L and Zhou, M and Stefano, G and Pandolfi, C and Mancuso, S and Shabala, S, Developing and validating a high-throughput assay for salinity tissue tolerance in wheat and barley, Planta, 242, (4) pp. 847-857. ISSN 0032-0935 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Springer-Verlag Berlin Heidelberg

DOI: doi:10.1007/s00425-015-2317-1

Abstract

A new high-throughput assay was developed to use the excised leaves to eliminate the confounding contribution of sodium exclusion mechanisms and evaluate genetic variability in salinity tissue tolerance in a large number of wheat (Triticum aestivum and Triticum turgidum ssp. durum) and barley (Hordeum vulgare) accessions. The changes in relative chlorophyll content (measured as chlorophyll content index, CCI) in excised leaves exposed to 50 mM NaCl for 48 h were found to be a reliable indicator of leaf tissue tolerance. In both wheat and barley, relative CCI correlated strongly with the overall plant salinity tolerance (evaluated in glasshouse experiments). To a large extent, this tissue tolerance was related to more efficient vacuolar Na+ sequestration in leaf mesophyll, as revealed by fluorescent Na+ dye imaging experiments. However, while in barley this correlation was positive, tissue tolerance in wheat correlated negatively with overall salinity tolerance. As a result, more salt-sensitive durum wheat genotypes possessed higher tissue tolerance than bread wheat plants, and this negative correlation was present within each of bread and durum wheat clusters as well. Overall, these results indicate that the lack of effective Na+ exclusion ability in sensitive wheat varieties is compensated by their better ability to handle Na+ accumulated in the shoot via tissue tolerance mechanisms. Implications of these findings for plant breeding for salinity tolerance are discussed.

Main conclusion: Leaf tissue tolerance was strongly and positively correlated with overall salt tolerance in barley, but not in wheat where the inability of sensitive varieties to exclude Na+ is compensated by their better ability to handle Na+ accumulated in the shoot via tissue tolerance mechanisms.

Item Details

Item Type:Refereed Article
Keywords:barley, chlorophyll content, excised leaf, tissue tolerance, vacuolar Na+ sequestration, wheat, Triticum aestivum, Triticum turgidum ssp. durum, Hordeum vulgare
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Wheat
Author:Wu, H (Mr Honghong Wu)
Author:Shabala, L (Dr Lana Shabala)
Author:Zhou, M (Professor Meixue Zhou)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:100732
Year Published:2015
Web of Science® Times Cited:5
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-05-27
Last Modified:2017-11-02
Downloads:0

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