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Calcium sensor kinase activates potassium uptake systems in gland cells of Venus flytraps

Citation

Scherzer, S and Bohm, J and Krol, E and Shabala, L and Kreuzer, I and Larisch, C and Bemm, F and Al-Rasheid, KAS and Shabala, S and Rennenberg, H and Neher, E and Hedrich, R, Calcium sensor kinase activates potassium uptake systems in gland cells of Venus flytraps, Proceedings of the National Academy of Sciences of The United States of America, 112, (23) pp. 7309-7314. ISSN 0027-8424 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 National Academy of Sciences

DOI: doi:10.1073/pnas.1507810112

Abstract

The Darwin plant Dionaea muscipula is able to grow on mineral-poor soil, because it gains essential nutrients from captured animal prey. Given that no nutrients remain in the trap when it opens after the consumption of an animal meal, we here asked the question of how Dionaea sequesters prey-derived potassium. We show that prey capture triggers expression of a K+ uptake system in the Venus flytrap. In search of K+ transporters endowed with adequate properties for this role, we screened a Dionaea expressed sequence tag (EST) database and identified DmKT1 and DmHAK5 as candidates. On insect and touch hormone stimulation, the number of transcripts of these transporters increased in flytraps. After cRNA injection of K+-transporter genes into Xenopus oocytes, however, both putative K+ transporters remained silent. Assuming that calcium sensor kinases are regulating Arabidopsis K+ transporter 1 (AKT1), we coexpressed the putative K+ transporters with a large set of kinases and identified the CBL9-CIPK23 pair as the major activating complex for both transporters in Dionaea K+ uptake. DmKT1 was found to be a K+-selective channel of voltage-dependent high capacity and low affinity, whereas DmHAK5 was identified as the first, to our knowledge, proton-driven, high-affinity potassium transporter with weak selectivity. When the Venus flytrap is processing its prey, the gland cell membrane potential is maintained around −120 mV, and the apoplast is acidified to pH 3. These conditions in the green stomach formed by the closed flytrap allow DmKT1 and DmHAK5 to acquire prey-derived K+, reducing its concentration from millimolar levels down to trace levels.

Item Details

Item Type:Refereed Article
Keywords:Dionaea muscipula, CIPK, HAK5, AKT, transporter
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
Author:Bohm, J (Ms Jennifer Bohm)
Author:Shabala, L (Dr Lana Shabala)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:100725
Year Published:2015
Web of Science® Times Cited:24
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-05-27
Last Modified:2017-11-06
Downloads:0

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