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Rapid regulation of the plasma membrane H+-ATPase activity is essential to salinity tolerance in two halophyte species, Atriplex lentiformis and Chenopodium quinoa

Citation

Bose, J and Rodrigo-Moreno, A and Lai, D and Xie, Y and Shen, W and Shabala, S, Rapid regulation of the plasma membrane H+-ATPase activity is essential to salinity tolerance in two halophyte species, Atriplex lentiformis and Chenopodium quinoa, Annals of Botany, 115, (3) pp. 481-494. ISSN 0305-7364 (2015) [Refereed Article]

Copyright Statement

© The Author 2014. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.

DOI: doi:10.1093/aob/mcu219

Abstract

Background and Aims The activity of H+-ATPase is essential for energizing the plasma membrane. It provides the driving force for potassium retention and uptake through voltage-gated channels and for Na+ exclusion via Na+/H+ exchangers. Both of these traits are central to plant salinity tolerance; however, whether the increased activity of H+-ATPase is a constitutive trait in halophyte species and whether this activity is upregulated at either the transcriptional or post-translation level remain disputed.

Methods The kinetics of salt-induced net H+, Na+ and K+ fluxes, membrane potential and AHA1/2/3 expression changes in the roots of two halophyte species, Atriplex lentiformis (saltbush) and Chenopodium quinoa (quinoa), were compared with data obtained from Arabidopsis thaliana roots.

Key Results Intrinsic (steady-state) membrane potential values were more negative in A. lentiformis and C. quinoa compared with arabidopsis (−144 ± 3·3, −138 ± 5·4 and −128 ± 3·3 mV, respectively). Treatment with 100 mm NaCl depolarized the root plasma membrane, an effect that was much stronger in arabidopsis. The extent of plasma membrane depolarization positively correlated with NaCl-induced stimulation of vanadate-sensitive H+ efflux, Na+ efflux and K+ retention in roots (quinoa > saltbush > arabidopsis). NaCl-induced stimulation of H+ efflux was most pronounced in the root elongation zone. In contrast, H+-ATPase AHA transcript levels were much higher in arabidopsis compared with quinoa plants, and 100 mm NaCl treatment led to a further 3-fold increase in AHA1 and AHA2 transcripts in arabidopsis but not in quinoa.

Conclusions Enhanced salinity tolerance in the halophyte species studied here is not related to the constitutively higher AHA transcript levels in the root epidermis, but to the plant’s ability to rapidly upregulate plasma membrane H+-ATPase upon salinity treatment. This is necessary for assisting plants to maintain highly negative membrane potential values and to exclude Na+, or enable better K+ retention in the cytosol under saline conditions.

Item Details

Item Type:Refereed Article
Keywords:AHA expression, Arabidopsis thaliana, Atriplex lentiformis, Chenopodium quinoa, H+ fluxes, K+ fluxes, Na+ fluxes, membrane potential, H+-ATPase, halophyte, salinity tolerance, saltbush
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Wheat
Author:Bose, J (Dr Jayakumar Bose)
Author:Rodrigo-Moreno, A (Ms Ana Rodrigo Moreno)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:100722
Year Published:2015
Web of Science® Times Cited:29
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-05-27
Last Modified:2016-05-06
Downloads:0

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