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MIFE technique-based screening for mesophyll K+ retention for crop breeding for salinity tolerance
Citation
Wu, H and Shabala, L and Zhou, M and Shabala, S, MIFE technique-based screening for mesophyll K+ retention for crop breeding for salinity tolerance, Bio-protocol, 5, (9) Article e1466. ISSN 2331-8325 (2015) [Contribution to Refereed Journal]
Copyright Statement
Copyright Bio-protocol 2015
Official URL: http://www.bio-protocol.org/e1466
Abstract
Potassium is known as a rate-limiting factor for crop yield and plays an important role in plants response under abiotic stresses. Recently, cytosolic K+ retention ability in leaf mesophyll has emerged as an important component of plant salt tolerance mechanism (Wu et al., 2013; Wu et al., 2014; Wu et al., 2015). In this protocol, the procedure for screening leaf mesophyll for K+ retention by the MIFE (microelectrode ion flux estimation) technique is described in detail using wheat as an example. By measuring NaCl-induced K+ efflux in leaf mesophyll, a large number of plant accessions can be screened and categorised according to their salinity stress tolerance. The method provides a rapid and reliable tool that targets the activity of specific membrane transporters directly contributing to salinity tolerance trait and, because of this, has a competitive advantage over traditional whole-plant phenotyping. While the focus of this protocol is on wheat, the suggested method may be adopted for screening K+ retention in leaf mesophyll in any other crop species.
Item Details
Item Type: | Contribution to Refereed Journal |
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Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Grains and seeds |
Objective Field: | Wheat |
UTAS Author: | Wu, H (Mr Honghong Wu) |
UTAS Author: | Shabala, L (Associate Professor Lana Shabala) |
UTAS Author: | Zhou, M (Professor Meixue Zhou) |
UTAS Author: | Shabala, S (Professor Sergey Shabala) |
ID Code: | 100720 |
Year Published: | 2015 |
Deposited By: | Tasmanian Institute of Agriculture |
Deposited On: | 2015-05-27 |
Last Modified: | 2016-08-02 |
Downloads: | 2 View Download Statistics |
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