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MIFE technique-based screening for mesophyll K+ retention for crop breeding for salinity tolerance

Citation

Wu, H and Shabala, L and Zhou, M and Shabala, S, MIFE technique-based screening for mesophyll K+ retention for crop breeding for salinity tolerance, Bio-protocol, 5, (9) Article e1466. ISSN 2331-8325 (2015) [Contribution to Refereed Journal]

Copyright Statement

Copyright Bio-protocol 2015

Official URL: http://www.bio-protocol.org/e1466

Abstract

Potassium is known as a rate-limiting factor for crop yield and plays an important role in plants response under abiotic stresses. Recently, cytosolic K+ retention ability in leaf mesophyll has emerged as an important component of plant salt tolerance mechanism (Wu et al., 2013; Wu et al., 2014; Wu et al., 2015). In this protocol, the procedure for screening leaf mesophyll for K+ retention by the MIFE (microelectrode ion flux estimation) technique is described in detail using wheat as an example. By measuring NaCl-induced K+ efflux in leaf mesophyll, a large number of plant accessions can be screened and categorised according to their salinity stress tolerance. The method provides a rapid and reliable tool that targets the activity of specific membrane transporters directly contributing to salinity tolerance trait and, because of this, has a competitive advantage over traditional whole-plant phenotyping. While the focus of this protocol is on wheat, the suggested method may be adopted for screening K+ retention in leaf mesophyll in any other crop species.

Item Details

Item Type:Contribution to Refereed Journal
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Wheat
Author:Wu, H (Mr Honghong Wu)
Author:Shabala, L (Dr Lana Shabala)
Author:Zhou, M (Associate Professor Meixue Zhou)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:100720
Year Published:2015
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-05-27
Last Modified:2016-08-02
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