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Linking salinity stress tolerance with tissue-specific Na+ sequestration in wheat roots
Citation
Wu, H and Shabala, L and Liu, X and Azzarello, E and Zhou, M and Pandolfi, C and Chen, Z-H and Bose, J and Mancuso, S and Shabala, S, Linking salinity stress tolerance with tissue-specific Na+ sequestration in wheat roots, Frontiers in Plant Science, 6 Article 71. ISSN 1664-462X (2015) [Refereed Article]
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Copyright Statement
Copyright 2015 Wu, Shabala, Liu, Azzarello, Zhou, Pandolfi, Chen, Bose, Mancuso and Shabala Licensed Under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/
DOI: doi:10.3389/fpls.2015.00071
Abstract
Salinity stress tolerance is a physiologically complex trait that is conferred by the large array of interacting mechanisms. Among these, vacuolar Na+ sequestration has always been considered as one of the key components differentiating between sensitive and tolerant species and genotypes. However, vacuolar Na+ sequestration has been rarely considered in the context of the tissue-specific expression and regulation of appropriate transporters contributing to Na+ removal from the cytosol. In this work, six bread wheat varieties contrasting in their salinity tolerance (three tolerant and three sensitive) were used to understand the essentiality of vacuolar Na+ sequestration between functionally different root tissues, and link it with the overall salinity stress tolerance in this species. Roots of 4-day old wheat seedlings were treated with 100 mM NaCl for 3 days, and then Na+ distribution between cytosol and vacuole was quantified by CoroNa Green fluorescent dye imaging. Our major observations were as follows: (1) salinity stress tolerance correlated positively with vacuolar Na+ sequestration ability in the mature root zone but not in the root apex; (2) contrary to expectations, cytosolic Na+ levels in root meristem were significantly higher in salt tolerant than sensitive group, while vacuolar Na+ levels showed an opposite trend. These results are interpreted as meristem cells playing a role of the "salt sensor;" (3) no significant difference in the vacuolar Na+ sequestration ability was found between sensitive and tolerant groups in either transition or elongation zones; (4) the overall Na+ accumulation was highest in the elongation zone, suggesting its role in osmotic adjustment and turgor maintenance required to drive root expansion growth. Overall, the reported results suggest high tissue-specificity of Na+ uptake, signaling, and sequestration in wheat roots. The implications of these findings for plant breeding for salinity stress tolerance are discussed.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | bread wheat, cytosolic Na+, Na+ distribution, root zones, salinity stress tolerance, vacuolar Na+ sequestration |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant physiology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Grains and seeds |
| Objective Field: | Barley |
| UTAS Author: | Wu, H (Mr Honghong Wu) |
| UTAS Author: | Shabala, L (Associate Professor Lana Shabala) |
| UTAS Author: | Zhou, M (Professor Meixue Zhou) |
| UTAS Author: | Bose, J (Dr Jayakumar Bose) |
| UTAS Author: | Shabala, S (Professor Sergey Shabala) |
| ID Code: | 100719 |
| Year Published: | 2015 |
| Web of Science® Times Cited: | 53 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2015-05-27 |
| Last Modified: | 2017-11-02 |
| Downloads: | 331 View Download Statistics |
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