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Fibulin1C peptide induces cell attachment and extracellular matrix deposition in lung fibroblasts

Citation

Ge, Q and Chen, L and Jaffar, J and Argaves, WS and Twal, WO and Hansbro, P, Fibulin1C peptide induces cell attachment and extracellular matrix deposition in lung fibroblasts, Scientific Reports, 5 Article 9496. ISSN 2045-2322 (2015) [Refereed Article]


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Copyright 2015 The Authors Licensed under a Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1038/srep09496

Abstract

Fibulin-1 is an extracellular matrix (ECM) protein, levels of which are elevated in serum and lung tissue from patients with idiopathic pulmonary fibrosis compared to healthy volunteers. Inhibition of fibulin-1C, one of four fibulin-1 isoforms, reduced proliferation and wound healing in human airway smooth muscle (ASM) cells. This study identified the bioactive region/s of fibulin-1C which promotes fibrosis. Seven fibulin-1C peptides were synthesized and used to pre-coat tissue culture plates before lung derived ASM cells and fibroblasts from patients with pulmonary fibrosis (PF), chronic obstructive pulmonary disease (COPD) or neither disease (Control) were plated. Peptide effects on in vitro measures of fibrosis: cell attachment, proliferation and viability, and ECM deposition, were examined. Among these peptides, peptide 1C1 (FBLN1C1) enhanced ASM cell and fibroblast attachment. FBLN1C1 increased mitochondrial activity and proliferation in fibroblasts. In addition, FBLN1C1 stimulated fibulin1 deposition in PF and COPD fibroblasts, and augmented fibronectin and perlecan deposition in all three groups. Peptides FBLN1C2 to FBLN1C7 had no activity. The active fibulin-1C peptide identified in this study describes a useful tool for future studies. Ongoing investigation of the role of fibulin-1 may reveal the mechanisms underlying the pathphysiology of chronic lung diseases.

Item Details

Item Type:Refereed Article
Keywords:Fibulin-1C; Extracellular Matrix; Lung; Fibroblast
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Respiratory Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Respiratory System and Diseases (incl. Asthma)
UTAS Author:Chen, L (Dr Ling Chen)
ID Code:100408
Year Published:2015
Web of Science® Times Cited:15
Deposited By:Medicine
Deposited On:2015-05-14
Last Modified:2017-11-02
Downloads:316 View Download Statistics

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