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Mating type distributions of Microsphaeropsis tanaceti isolated from pyrethrum fields in Tasmania, Australia
Citation
Pearce, TL and Scott, JB and Pethybridge, SJ and Hay, FS, Mating type distributions of Microsphaeropsis tanaceti isolated from pyrethrum fields in Tasmania, Australia, 2014 APS-CPS Joint Meeting - Abstracts of Presentations, 9-13 August, Minneapolis, Minnesota, pp. S3.90. (2014) [Conference Extract]
 | PDF (Pearce, T. L., Scott, J. B., Pethybridge, S. J., and Hay, F. S. 2014. Mating type distributions of Microsphaeropsis tanaceti isolated from pyrethrum fields in Tasmania, Australia. Phytopathology 104:S3.90) Pending copyright assessment - Request a copy 2Mb |
Abstract
Tan spot disease, caused by the ascomycete Microsphaeropsis tanaceti, is a
prominent disease in Tasmanian pyrethrum crops, which are grown for
insecticide production. M. tanaceti reproduces profusely via asexual conidia,
however ascospores haven’t been observed and the role of sexual reproduction
in the field unknown. In many ascomycete fungi, mating type (MAT) genes
govern mating between individuals. In this study, two mating types, MAT1-1
and MAT1-2, have been identified within M. tanaceti in a heterothallic
arrangement. A PCR assay was developed to identify mating type and used to
screen two hierarchically sampled, field populations in August 2012. Each
field consisted of two 50 m transects, sampled at 0.5 m intervals (n = 101 per
transect). Within each transect, M. tanaceti was isolated from 62 and 46
sampling units in field A, and 21 and 45 sampling units in field B. The ratio of
mating types did not differ from a 1:1 ratio at either the field or transect scale
(P ≥ 0.13). Ordinary runs analysis identified no spatial aggregation of M.
tanaceti or of MAT1-2 genotypes (P ≥ 0.06) within transects. Spatially
significant (P < 0.05) aggregation of MAT1-1 was only identified in a single
transect in field B. This data indicates a heterothallic species with no evidence
to preclude a sexual cycle occurring in the field. MAT gene functionality will
be examined via in vitro crosses and RNA expression analysis.
Item Details
| Item Type: | Conference Extract |
|---|---|
| Keywords: | pyrethrum, tan spot, population genetics |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant pathology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Horticultural crops |
| Objective Field: | Horticultural crops not elsewhere classified |
| UTAS Author: | Pearce, TL (Dr Tamieka Pearce) |
| UTAS Author: | Scott, JB (Dr Jason Scott) |
| UTAS Author: | Pethybridge, SJ (Dr Sarah Pethybridge) |
| UTAS Author: | Hay, FS (Dr Frank Hay) |
| ID Code: | 100315 |
| Year Published: | 2014 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2015-05-11 |
| Last Modified: | 2015-05-13 |
| Downloads: | 0 |
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