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Novel method for conscious airway resistance and ventilation estimation in neonatal rodents using plethysmography and a mechanical lung

Citation

Zhang, B and McDonald, FB and Cummings, KJ and Frappell, P and Wilson, RJA, Novel method for conscious airway resistance and ventilation estimation in neonatal rodents using plethysmography and a mechanical lung, Respiratory Physiology and Neurobiology, 201 pp. 75-83. ISSN 1569-9048 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Elsevier B.V.

DOI: doi:10.1016/j.resp.2014.07.004

Abstract

In unrestrained whole body plethysmography, tidal volume is commonly determined using the barometric method, which assumes that temperature and humidity changes (the 'barometric component') are solely responsible for breathing-related chamber pressure fluctuations. However, in small animals chamber pressure is also influenced by a 'mechanical component' dependent on airway resistance and airflow. We devised a novel 'mechanical lung' capable of simulating neonatal mouse breathing in the absence of temperature or humidity changes. Using this device, we confirm that the chamber pressure fluctuations produced by breathing of neonatal mice are dominated by the mechanical component, precluding direct quantitative assessment of tidal volume. Recognizing the importance of airway resistance to the chamber pressure signal and the ability of our device to simulate neonatal breathing at different frequencies and tidal volumes, we invented a novel in vivo, non-invasive method for conscious airway resistance and ventilation estimation (CARVE) in neonatal rodents. This technique will allow evaluation of developmental, pathological and pharmaceutical effects on airway resistance.

Item Details

Item Type:Refereed Article
Keywords:plethysmography, neonatal mouse, barometric method, tidal volume, airway resistance, mechanical lung
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Vertebrate Biology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Frappell, P (Professor Peter Frappell)
ID Code:100305
Year Published:2014
Web of Science® Times Cited:3
Deposited By:Research Division
Deposited On:2015-05-11
Last Modified:2015-06-17
Downloads:0

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