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Novel method for conscious airway resistance and ventilation estimation in neonatal rodents using plethysmography and a mechanical lung
journal contribution
posted on 2023-05-18, 09:47 authored by Zhang, B, McDonald, FB, Cummings, KJ, Peter FrappellPeter Frappell, Wilson, RJAIn unrestrained whole body plethysmography, tidal volume is commonly determined using the barometric method, which assumes that temperature and humidity changes (the 'barometric component') are solely responsible for breathing-related chamber pressure fluctuations. However, in small animals chamber pressure is also influenced by a 'mechanical component' dependent on airway resistance and airflow. We devised a novel 'mechanical lung' capable of simulating neonatal mouse breathing in the absence of temperature or humidity changes. Using this device, we confirm that the chamber pressure fluctuations produced by breathing of neonatal mice are dominated by the mechanical component, precluding direct quantitative assessment of tidal volume. Recognizing the importance of airway resistance to the chamber pressure signal and the ability of our device to simulate neonatal breathing at different frequencies and tidal volumes, we invented a novel in vivo, non-invasive method for conscious airway resistance and ventilation estimation (CARVE) in neonatal rodents. This technique will allow evaluation of developmental, pathological and pharmaceutical effects on airway resistance.
History
Publication title
Respiratory Physiology and NeurobiologyVolume
201Pagination
75-83ISSN
1569-9048Department/School
Institute for Marine and Antarctic StudiesPublisher
Elsevier Science BvPlace of publication
PO Box 211, Amsterdam, Netherlands, 1000 AeRights statement
Copyright 2014 Elsevier B.V.Repository Status
- Restricted