eCite Digital Repository
Analysis of melamine in milk powder and liquid milk by capillary zone electrophoresis after electrokinetic supercharging
Citation
Lu, Y and Wang, D and Kong, C and Jia, C and Breadmore, MC, Analysis of melamine in milk powder and liquid milk by capillary zone electrophoresis after electrokinetic supercharging, Food Analytical Methods, 8, (5) pp. 1356-1362. ISSN 1936-9751 (2015) [Refereed Article]
Copyright Statement
Copyright 2014 Springer Science+Business Media New York
DOI: doi:10.1007/s12161-014-9987-3
Abstract
A method was developed to determine melamine in milk powder and liquid milk by capillary zone electrophoresis after electrokinetic supercharging. The effects of buffer acidity and concentration, the type and length of leader and terminator electrolytes, injection time and matrix effects were investigated. The optimum background electrolyte was 50 mM sodium citrate-citric acid (pH 4.5), hydrodynamic injection of 50 mM sodium chloride (24 s, 0.5 psi) as leader, electrokinetic injection of the sample (100 s, 10 kV), hydrodynamic injection of 50 mM tetrabutylammonium bromide (18 s, 0.5 psi) as terminator and then a voltage of 25 kV for separation (+25 kV). Under these conditions, the sensitivity was enhanced 2285 times when compared to a normal hydrodynamic injection with the sample volume <3 % of the capillary volume. The detection limit for melamine with this electrokinetic supercharging (EKS) system is 0.7 ng/mL. The developed method was verified by analysing milk powder and liquid milk samples, and the recovery was 29.8 and 42.1 %, respectively.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | food, capillary zone electrophoresis, electrokinetic supercharging, milk powder, liquid milk |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Breadmore, MC (Professor Michael Breadmore) |
ID Code: | 100262 |
Year Published: | 2015 |
Funding Support: | Australian Research Council (FT130100101) |
Web of Science® Times Cited: | 8 |
Deposited By: | Chemistry |
Deposited On: | 2015-05-08 |
Last Modified: | 2017-11-02 |
Downloads: | 0 |
Repository Staff Only: item control page