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Using reaction flow chromatography for the analysis of amino acid: derivatisation with fluorescamine reagent


Pravadali-Cekic, S and Jones, A and Kazarian, AA and Paull, B and Soliven, A and Ritchie, H and Camenzuli, M and Dennis, GR and Shalliker, RA, Using reaction flow chromatography for the analysis of amino acid: derivatisation with fluorescamine reagent, Microchemical Journal, 121 pp. 141-149. ISSN 0026-265X (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Elsevier B.V.

DOI: doi:10.1016/j.microc.2015.02.010


Reaction flow (RF) chromatography with fluorescamine reagent and UV–vis detection was used for the analysis of amino acids. The performance advantage of RF chromatography was tested against the conventional post-column derivatisation (PCD) methods. The results of the study verified that greater sensitivity could be obtained using RF columns compared to conventional PCD methods. The reaction loops employed in the conventional PCD methods did not need to be used, which resulted in more efficient separations using RF columns. The selective detection of amino acids in this RF study reduced limits of detection (LOD) and limits of quantification (LOQ) by about 50% compared to conventional methods of PCD. The calibration data of the RF-PCD technique also yielded R2 ≥ 0.99 and %RSD in peak areas ranging from 3.0–8.5%. The RF method also enabled multiplexed detection since both the derivatised and non-derivatised flow streams could be analysed simultaneously.

Item Details

Item Type:Refereed Article
Keywords:amino acids, post-column derivatisation, reaction flow chromatography, fluorescamine
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Kazarian, AA (Dr Tom Kazarian)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:100247
Year Published:2015
Web of Science® Times Cited:11
Deposited By:Chemistry
Deposited On:2015-05-07
Last Modified:2017-10-29

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