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Using reaction flow chromatography for the analysis of amino acid: derivatisation with fluorescamine reagent
Citation
Pravadali-Cekic, S and Jones, A and Kazarian, AA and Paull, B and Soliven, A and Ritchie, H and Camenzuli, M and Dennis, GR and Shalliker, RA, Using reaction flow chromatography for the analysis of amino acid: derivatisation with fluorescamine reagent, Microchemical Journal, 121 pp. 141-149. ISSN 0026-265X (2015) [Refereed Article]
Copyright Statement
Copyright 2015 Elsevier B.V.
DOI: doi:10.1016/j.microc.2015.02.010
Abstract
Reaction flow (RF) chromatography with fluorescamine reagent and UV–vis detection was used for the analysis of amino acids. The performance advantage of RF chromatography was tested against the conventional post-column derivatisation (PCD) methods. The results of the study verified that greater sensitivity could be obtained using RF columns compared to conventional PCD methods. The reaction loops employed in the conventional PCD methods did not need to be used, which resulted in more efficient separations using RF columns. The selective detection of amino acids in this RF study reduced limits of detection (LOD) and limits of quantification (LOQ) by about 50% compared to conventional methods of PCD. The calibration data of the RF-PCD technique also yielded R2 ≥ 0.99 and %RSD in peak areas ranging from 3.0–8.5%. The RF method also enabled multiplexed detection since both the derivatised and non-derivatised flow streams could be analysed simultaneously.
Item Details
Item Type: | Refereed Article |
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Keywords: | amino acids, post-column derivatisation, reaction flow chromatography, fluorescamine |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Kazarian, AA (Dr Tom Kazarian) |
UTAS Author: | Paull, B (Professor Brett Paull) |
ID Code: | 100247 |
Year Published: | 2015 |
Web of Science® Times Cited: | 11 |
Deposited By: | Chemistry |
Deposited On: | 2015-05-07 |
Last Modified: | 2017-10-29 |
Downloads: | 0 |
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