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Using reaction flow chromatography for the analysis of amino acid: derivatisation with fluorescamine reagent
journal contribution
posted on 2023-05-18, 09:41 authored by Pravadali-Cekic, S, A Jones, Kazarian, AA, Brett PaullBrett Paull, Soliven, A, Ritchie, H, Camenzuli, M, Dennis, GR, Shalliker, RAReaction flow (RF) chromatography with fluorescamine reagent and UV–vis detection was used for the analysis of amino acids. The performance advantage of RF chromatography was tested against the conventional post-column derivatisation (PCD) methods. The results of the study verified that greater sensitivity could be obtained using RF columns compared to conventional PCD methods. The reaction loops employed in the conventional PCD methods did not need to be used, which resulted in more efficient separations using RF columns. The selective detection of amino acids in this RF study reduced limits of detection (LOD) and limits of quantification (LOQ) by about 50% compared to conventional methods of PCD. The calibration data of the RF-PCD technique also yielded R2 ≥ 0.99 and %RSD in peak areas ranging from 3.0–8.5%. The RF method also enabled multiplexed detection since both the derivatised and non-derivatised flow streams could be analysed simultaneously.
History
Publication title
Microchemical JournalVolume
121Pagination
141-149ISSN
0026-265XDepartment/School
School of Natural SciencesPublisher
Elsevier Science BvPlace of publication
Po Box 211, Amsterdam, Netherlands, 1000 AeRights statement
Copyright 2015 Elsevier B.V.Repository Status
- Restricted