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Development of a silica monolith modified with Fe3O4 nano-particles in centrifugal spin column format for the extraction of phosphorylated compounds
Citation
Alwy, A and Clarke, SP and Brougham, DF and Twamley, B and Paull, B and White, B and Connolly, D, Development of a silica monolith modified with Fe3O4 nano-particles in centrifugal spin column format for the extraction of phosphorylated compounds, Journal of Separation Science, 38, (2) pp. 283-290. ISSN 1615-9306 (2015) [Refereed Article]
Copyright Statement
Copyright 2014 WILEY-VCH Verlag GmbH & Co
DOI: doi:10.1002/jssc.201400924
Abstract
In this study, citrate-stabilised iron oxide nano-particles (∼16 nm) have been immobilised on commercial silica monolithic centrifugal spin columns (MonoSpin) for the extraction of phosphorylated compounds. Two alternative strategies were adopted involving either direct electrostatic attachment to an aminated MonoSpin (single-layer method) in the first instance, or the use of a layer-by-layer method with poly(diallyldimethylammonium) chloride. Field-emission scanning electron spectroscopy and energy-dispersive X-ray spectroscopy was used for confirming notably higher coverage of nano-particles using the layer-by-layer method (2.49 ± 0.53 wt%) compared with the single-layer method (0.43 ± 0.30 wt%). The modified monolith was used for the selective separation/extraction of adenosine monophosphate, adenosine diphosphate and adenosine triphosphate with elution using a phosphate buffer. A reversed-phase liquid chromatographic assay was used for confirming that adenosine, as a non-phosphorylated control was not retained on the modified MonoSpin devices, whereas recovery of 80% for adenosine monophosphate, 86% for adenosine diphosphate and 82% for adenosine triphosphate was achieved.
Item Details
Item Type: | Refereed Article |
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Keywords: | centrifugal spin columns, iron oxide, nano particles, nucleotide |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Paull, B (Professor Brett Paull) |
ID Code: | 100222 |
Year Published: | 2015 |
Web of Science® Times Cited: | 9 |
Deposited By: | Chemistry |
Deposited On: | 2015-05-07 |
Last Modified: | 2017-10-28 |
Downloads: | 0 |
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