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Carpogenic germination of sclerotia of Sclerotinia minor and ascosporic infection of pyrethrum flowers

Citation

O'Malley, TB and Hay, FS and Scott, JB and Gent, DH and Shivas, RG and Pethybridge, SJ, Carpogenic germination of sclerotia of Sclerotinia minor and ascosporic infection of pyrethrum flowers, Canadian Journal of Plant Pathology, 37, (2) pp. 179-187. ISSN 0706-0661 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 The Canadian Phytopathological Society

DOI: doi:10.1080/07060661.2015.1036122

Abstract

Carpogenic germination of sclerotia and infection of flowers by ascospores of Sclerotinia minor is rare and seldom documented in most hosts. During 20072009, S. minor isolates were obtained from surface-sterilized pyrethrum flowers collected from fields in Australia. The isolation frequency of S. minor from flowers in 2007, 2008 and 2009 was 15.8%, 5% and 1.4%, respectively. During these years, the prevalence of S. minor in flowers amongst pyrethrum fields varied between 10.3% and 60%. Sclerotia with apothecia, consistent in size with S. minor, were collected in one field. Colonies from individual ascospores from this isolate were identified as S. minor. A subsample of 10 S. minor isolates was selected for further studies. Phylogenetic analysis based on the internal transcribed spacer region grouped these isolates with S. minor, and distinct from published sequences of other Sclerotinia spp. Species-specific primers developed previously to differentiate the four major Sclerotinia spp. (S. sclerotiorum, S. minor, S. homoeocarpa and S. trifoliorum) were used to confirm identity. Of the 10 S. minor isolates, eight were able to carpogenically germinate in vitro. Pathogenicity of S. minor to flowers was confirmed in the greenhouse using ascospores. This study is one of the few instances documenting the ability of S. minor to infect floral tissues and the first documentation of S. minor causing flower disease of pyrethrum. These findings serve as a scaffold for further investigations into the mechanisms of flower infection by S. minor and have implications for the management of the Sclerotinia disease complex affecting pyrethrum in Australia.

Item Details

Item Type:Refereed Article
Keywords:Sclerotinia minor, ascospore, pyrethrum, carpogenic germination, pyrethrum, Sclerotinia flower blight, Sclerotinia sclerotiorum
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Pathology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Industrial Crops
Objective Field:Plant Extract Crops (e.g. Pyrethrum, Jojoba)
UTAS Author:O'Malley, TB (Mr Thomas O'Malley)
UTAS Author:Hay, FS (Dr Frank Hay)
UTAS Author:Scott, JB (Dr Jason Scott)
ID Code:100051
Year Published:2015
Web of Science® Times Cited:1
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-04-27
Last Modified:2017-11-02
Downloads:0

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